Brevibacterium fuscum protocatechuate 3,4-dioxygenase. Purification, crystallization, and characterization.
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چکیده
منابع مشابه
Purification and crystallization of 2,3-dihydroxybiphenyl 1,2-dioxygenase.
2,3-Dihydroxybiphenyl 1,2-dioxygenase, an enzyme of the biphenyl biodegradation pathway that cleaves the first of the aromatic rings, was purified to apparent homogeneity from Pseudomonas sp. strain LB400 that had been engineered to hyperexpress the bphC gene. The enzyme had a subunit molecular mass of 33.2 kDa as determined by SDS-polyacrylamide electrophoresis. Kinetic studies indicate a KM o...
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متن کاملReaction mechanism of protocatechuate 3,4-dioxygenase.
We have observed that high SOD-like function (decomposition of superoxide anion) was observed for several iron(III) compounds with tripodal ligands and several oxovanadium(IV) compounds, and also that these compounds exhibit high catalytic activity for oxidative cleavage of 3,5-di-tert-butylcatechol in non-donating solvents such as dichloromethane or nitromethane. These are suggesting that the ...
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We show here that purified chlorocatechol dioxygenase from Pseudomonas putida is able to oxygenate a wide range of substituted catechols with turnover numbers ranging from 2 to 29 s-1. This enzyme efficiently cleaves substituted catechols bearing electron-donating or multiple electron-withdrawing groups in an intradiol manner with kcat/KM values between 0.2 x 10(7) and 1.4 x 10(7) M-1 s-1. Thes...
متن کاملOxygenated form of protocatechuate 3,4-dioxygenase, a non-heme iron-containing dioxygenase, as reaction intermediate.
A short-lived new spectral species of protocatechuate 3,4-dioxygenase, a trivalent non-heme iron-containing enzyme, was observed in the early stage of the reaction. This new spectral species was characterized by a broad absorption band with a maximum between 500 and 520 rnp, distinct from those of the enzyme or the enzyme-protocatechuic acid complex. It could be demonstrated only in the presenc...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1984
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)43071-7